Abstract

A surface scanning technique was used to obtain resonant Raman spectra from chlorophylls in solution and in whole cells of diatoms at 80 K. When <i>in vivo</i> samples are excited at 457.9 nm, chlorophyll <i>c</i> becomes observable in the recorded Raman spectra, as shown by comparison with <i>in vitro</i> measurements. In the field of photosynthetic research this becomes interesting, as chlorophyll <i>c</i> can hardly be detected in electronic absorption spectra from whole cells, even at low temperatures, because of its weak Q bands compared with other plant chlorophylls, Chl <i>a</i> spectra were obtained with the use of 441.6-nm excitation. Working with two-channel recording, we eliminated the solvent contribution from the Raman spectra of pigments <i>in vitro.</i> Moreover, the technique facilitates detection of Raman excitation profiles from pigment lines <i>in vivo</i> at low temperatures by use of a nonresonating reference in the second sample chamber.

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