Nuclei extracted from lymphocytes coming from normal subjects and from patients affected by chronic lymphatic leukemia (B-CLL) were studied by means of infrared spectroscopy. Substantial spectral differences were found above all in the region of the phosphate-group vibrations of DNA. The ratios of the integrated areas of the bands at 1080 and 1540 cm<sup>−1</sup>, due to the symmetrical stretching vibrations of PO<sub>2</sub><sup>−</sup> groups and to proteic components, respectively, assume increasing values, which are localized in quite separate ranges, for normal lymphocytes, leukemic lymphocytes, normal nuclei, and leukemic nuclei. These values, indicating a different distribution of DNA and of proteic components between normal and leukemic cells and between normal and leukemic nuclei, may assume a remarkable significance in the precocious diagnosis of leukemia (B-CLL) and in following the course of the disease. In the case of leukemic nuclei, the ratio, in weight, of the two components reaches the value of 1:1.

PDF Article

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription