Abstract

A cause of deviation from Beer's Law is discovered. It is the presence of excess indicator in the standard solutions of the calibration curve when the indicator absorbs. The deviation is negative with either solvent or blank-reference. Theoretical and experimental evidences are given.

In Defense of Beer’s Law

Arthur C. Hardy and F. Mansfield Young
J. Opt. Soc. Am. 38(10) 854-858 (1948)

Beer’s Law and the Optimum Transmittance in Absorption Measurements

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Appl. Opt. 2(9) 937-945 (1963)

Deviations of Lambert-Beer’s law affect corneal refractive parameters after refractive surgery

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Correction to the Beer-Lambert-Bouguer law for optical absorption

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Noise reduction from chromophore images and reliability improvement by successive minimization of intermixture in the modified Lambert-Beer law

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