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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 35,
  • Issue 5,
  • pp. 461-465
  • (1981)

Fourier Transform Infrared Spectra of Calf Thymus DNA and its Reactions with the Anticancer Drug Cisplatin

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Abstract

Fourier transform infrared (FT-IR) spectra of deoxyribonucleic acid (DNA) and its reaction products with the anticancer drug <i>cis</i>-Pt(NH<sub>3</sub>)<sub>2</sub>Cl<sub>2</sub>, <i>cis</i>-diamminodichloroplatinum (abbreviated cisplatin) in water and heavy water solutions or gels have been obtained and studied. The mid-IR spectra show considerable changes in the carbonyl (C==O) region and weaker, but significant perturbations in the sugar-phosphate absorption region (PO<sub>2</sub><sup>−</sup>, OPC and COP). The carbonyl bands at 1710 and 1686 cm<sup>−1</sup> of the control DNA in H<sub>2</sub>O and D<sub>2</sub>O, respectively, disappear and shift to lower frequencies in the spectra of cisplatin + DNA with a <i>r</i> = Pt/P = 0.2. Furthermore, the sugar-phosphate band at 1054 cm<sup>−1</sup> of the C—O stretching vibration of the ribose-phosphate bond is reduced in intensity and slightly shifted to higher frequencies, indicating a reorientation of the phosphate group. In addition, the P—O stretching vibration frequency at 970 cm<sup>−1</sup> is reduced in intensity on interaction with the drug. These changes depend to some extent on the water content in the control DNA and in the cisplatin + DNA complex. The water molecules in DNA in the presence of cisplatin reorganize themselves. The attack of the platinum drug at the N<sub>7</sub> site of the guanine molecule in DNA exerts an effect on the DNA structure through a modification of the water average configuration and its stabilizing effect on the secondary structure.

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