Abstract

Experimentally determined Raman excitation profiles are reported for adenosine monophosphate (AMP), adenylyl-(3' → 5')-adenosine, and polyadenylic acid. Excitation in the ultraviolet is restricted to the preresonance region to avoid the necessity for inclusion of linewidth factors in the theoretical fit to the experimental data. The effective absorption wavelengths from which the three most intense vibrations derive their intensities are determined. For each vibrational mode, no appreciable difference in the excitation profiles of each of these systems is observed, but the results for AMP are found to differ from those previously reported. The utility of these measurements with respect to conformational studies is discussed.

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