The electron paramagnetic resonance signal of the cupric ion (its perpendicular component) at −196°C was found to be different in shape in human blood plasma and serum. On the addition of acid, substrate of ceruloplasmin, reducing agent, or denaturing agent, serum exhibited a complex electron paramagnetic resonance spectrum similar to that of a copper-diethyldithiocarbamate complex in an organic solvent, with a simultaneous disappearance of the cupric ion signal. Such an electron paramagnetic resonance spectrum occurred only from human serum but not from animal (guinea pig, mouse, rat, cattle, or monkey) serum.

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