Abstract

A small volume, self-contained, anaerobic circulating flow cell has been developed and applied to the resonance Raman investigation of two heme proteins: carboxyhemoglobin and cytochrome <i>c</i>. The cell has been demonstrated to be effective in reducing photodissociation of light-sensitive compounds. The flow performance is competitive with several alternative approaches. Electrochemical methods have been coupled with visible absorption and resonance Raman spectroscopy to titrate and determine potentiometrically the formal reduction potential of cytochrome <i>c.</i> Agreement between the results of visible and Raman spectral data demonstrates the feasibility of utilizing quantitative resonance Raman spectroscopy as a probe of formal reduction potentials of enzyme components.

Simultaneous measurement of changes in light absorption due to the reduction of cytochrome c oxidase and light scattering in rat brains during loss of tissue viability

Satoko Kawauchi, Shunichi Sato, Hidetoshi Ooigawa, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi
Appl. Opt. 47(22) 4164-4176 (2008)

Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations

Sean P. O’Connor, Samantha M. Powell, John M. Rickman, Nathaniel J. Pope, Gary D. Noojin, Marlan O. Scully, Michael L. Denton, and Vladislav V. Yakovlev
Biomed. Opt. Express 12(11) 7082-7091 (2021)

Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source

Scott R. Domingue, Randy A. Bartels, Adam J. Chicco, and Jesse W. Wilson
Biomed. Opt. Express 8(6) 2807-2821 (2017)

Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain

Y. Sakata, M. Abajian, M. O. Ripple, and R. Springett
Biomed. Opt. Express 3(8) 1933-1946 (2012)

Transient absorption spectroscopy and imaging of redox in muscle mitochondria

Erkang Wang, Luke A. Whitcomb, Adam J. Chicco, and Jesse W. Wilson
Biomed. Opt. Express 13(4) 2103-2116 (2022)