Abstract

The design and construction of a computer-controlled Raman spectrophotometer for the determination of difference spectra among up to four samples is described. The advantages of control by a minicomputer compared to a microcomputer or a hard wired controller are discussed. Conditions for the attainment of good signal/noise ratios with aqueous solutions of nucleotides in the 1 to 25 m<i>M</i> range are examined for the case of excitation in the visible spectrum to minimize radiation damage. Applications to studies of dilute aqueous solutions of biological molecules are discussed. The interactions of small molecules with biopolymers, particularly, are examined.

Digital Laser Raman Spectrometer System with On-Line Computer Control of Data Acquisition and Reduction

Charles H. Warren and Louis Ramaley
Appl. Opt. 12(8) 1976-1982 (1973)

On-Line Acquisition of Data from Raman and Infrared Spectrometers with a Time-Sharing Computer

J. R. Scherer and S. Kint
Appl. Opt. 9(7) 1615-1622 (1970)

On-line data reduction with a prism spectrometer

S. Loughin, C. Y. Yang, and J. E. Fischer
Appl. Opt. 14(6) 1373-1379 (1975)

Computer controlled cw laser spectrometer

C. R. Pollock, J. Kasper, G. K. Ernst, W. E. Ernst, S. Blit, and F. K. Tittel
Appl. Opt. 18(12) 1907-1912 (1979)

Photoconductivity study of ZnSiP₂ and CdSe:Cu by a computer-controlled spectrometer

Daniel Barreto, Jorge Luengo, Yovanina De Vita, and N. V. Joshi
Appl. Opt. 26(24) 5280-5283 (1987)