Abstract
The fluorescence characteristics of hematoporphyrin have been studied at 26°C as a function of concentration and pH. The pk values are 4.7 and 8.2 as determined in water and water-ethanol mixtures. The fluorescence spectrum consists of a peak at 625 nm and a second, less intense band at 675 nm. These emission bands are assigned to the Qy (0 → 0) and Q<sub>x</sub> (0 → 0) states. The four absorption bands in the visible region are assigned to π → π* transitions as follows; 502 nm, (1 ← 0); 536 nm, Qy (0 ← 0); 557 nm, Qx (1 ← 0); and 605 nm, Qx (0 ← 0). Corresponding excitation wavelengths include 505, 540, 570, and 620 nm. Polarized fluorescence is used to establish molecular association as a function of concentration. The polarization factor is used to verify the change from D<sub>2</sub><sub>h</sub> to D<sub>4</sub><sub>h</sub> symmetry as a function of pH.
PDF Article
More Like This
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription