Abstract

Fluorescence data of replicate samples obtained from different fluorescence spectrometers or by the same spectrometer but with different instrument settings can have great intensity differences. In order to compare such data an intensity calibration must be applied. Here we explain a simple calibration method for fluorescence intensity using only the integrated area of a water Raman peak. By applying this method to data from three different instruments, we show that it is possible to remove instrument-dependent intensity factors, and we present results on a unified scale of Raman units. The method presented is a rapid and simple approach suitable for routine measurements with no need for hazardous chemicals.

Effect of polarization and geometric factors on quantitative laser-induced fluorescence- to-Raman intensity ratios of water samples and a new calibration technique

Vasanthi Sivaprakasam and Dennis K. Killinger
J. Opt. Soc. Am. B 20(9) 1980-1989 (2003)

Development and initial calibration of a portable laser-induced fluorescence system used for in situ measurements of trace plastics and dissolved organic compounds in seawater and the Gulf of Mexico

Vasanthi Sivaprakasam, Robert F. Shannon, Caiyan Luo, Paula G. Coble, Jennifer R. Boehme, and Dennis K. Killinger
Appl. Opt. 42(33) 6747-6756 (2003)

Absolute calibration of a fluorescence collection system by Raman scattering in H₂

William K. Bischel, Douglas J. Bamford, and Leonard E. Jusinski
Appl. Opt. 25(7) 1215-1221 (1986)

Raman scattering by pure water and seawater

Jasmine S. Bartlett, Kenneth J. Voss, Shubha Sathyendranath, and Anthony Vodacek
Appl. Opt. 37(15) 3324-3332 (1998)

Standardization of Fluorescence Spectra and the Calibration of Spectrofluorimeters

J. W. Eastman
Appl. Opt. 5(7) 1125-1132 (1966)