Abstract

The authors describe the use of an ultraviolet light-emitting diode (UV LED), Nichia NSHU590E, as a 373 nm excitation light source for fluorescence lifetime measurements. A frequency-domain technique has been chosen to demonstrate that the UV LED can be efficiently intensity modulated by driving the radio-frequency signal over a range of modulation frequencies from 10 kHz up to 330 MHz. Time-resolved measurements have been performed by using a fluorophore with a very long lifetime of 10.2 mu s and fluorophore with a very short lifetime of 0.15 ns. Application of LEDs for lifetime-based chemical sensing is discussed.

Use of nanosecond excitation pulses in fluorescence lifetime measurement via phasor analysis

Dongeun Kim, Sucbei Moon, Wonsang Hwang, and Dug Young Kim
Opt. Express 30(9) 14677-14685 (2022)

Frequency-domain fluorescence lifetime optrode system design and instrumentation without a concurrent reference light-emitting diode

Mohammad Rameez Chatni, Gang Li, and David Marshall Porterfield
Appl. Opt. 48(29) 5528-5536 (2009)

Stability design of a light-emitting diode light source for trace constituent concentration measurement

Ling Ma, Fajie Duan, Jiajia Jiang, Xiao Fu, Tingting Huang, and Jingtao Chen
Appl. Opt. 56(36) 10046-10054 (2017)

Enhancement of light output power in GaN-based light-emitting diodes using hydrothermally grown ZnO micro-walls

Hyun Jeong, Yong Hwan Kim, Tae Hoon Seo, Hong Seok Lee, Jun Sung Kim, Eun-Kyung Suh, and Mun Seok Jeong
Opt. Express 20(10) 10597-10604 (2012)

Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode

V. Poher, H. X. Zhang, G. T. Kennedy, C. Griffin, S. Oddos, E. Gu, D. S. Elson, J. M. Girkin, P.M.W. French, M.D. Dawson, and M.A.A. Neil
Opt. Express 15(18) 11196-11206 (2007)