Abstract

For the acquisition of high-quality ultraviolet resonance Raman spectra of strongly scattering samples such as membrane protein suspensions, an f /3.5 Littrow prism prefilter has been designed, built, and characterized. This prefilter has a Czerny-Turner configuration, and its focal length is 25 cm. The apex angle of the dispersive prism (20 ) was chosen to provide maximum performance in the 220 to 240 nm range. The prism prefilter significantly reduced stray background due to Rayleigh scattering and visible fluorescence, while maintaining a low dispersion of 1300 cm-1/mm at 253 nm as well as a large fl /3.5 aperture. The sharpness of the transmission edge (at 242 nm, the T = 0% to 95% transition occurs in 1.3 nm) quantitates its effectiveness as a sharp-cut Rayleigh scattering filter. The total throughput of the prefilter is ~60% at 235 nm and 50% at 632.8 nm. The utility of this prefilter is demonstrated by obtaining high signal-to-noise resonance Raman spectra of bacteriorhodopsin in a purple membrane suspension with 239.5 nm excitation.

Kaleidoscope modes in large aperture Porro prism resonators

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Hyper-Rayleigh light scattering from an aqueous suspension of purple membrane

P. K. Schmidt and G. W. Rayfield
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Multipass prism monochromator for coherent Raman spectroscopy

Thomas L. Gaussiran, Roger H. Taylor, James L. Higdon, and J. W. Keto
Appl. Opt. 28(9) 1657-1660 (1989)

Highly efficient and aberration-corrected spectrometer for advanced Raman spectroscopy

Masayuki Futamata, Takehiko Takenouchi, and Kei-ichi Katakura
Appl. Opt. 41(22) 4655-4665 (2002)

Rational design of fiber-optic probes for visible and pulsed-ultraviolet resonance Raman spectroscopy

L. Shane Greek, H. Georg Schulze, Charles A. Haynes, Michael W. Blades, and Robin F. B. Turner
Appl. Opt. 35(21) 4086-4095 (1996)