Step-scan FT-IR difference spectroscopy with 100-ns time resolution is applied to the membrane protein bacteriorhodopsin. The experimetal setup and data acquisition are described in great detail. The IR absorbance changes accompanying bacteriorhodopsin's photocycle are presented in a three-dimensional representation. The data quality allows one, for example, to resolve the biphasic rise of the photocycle-intermediate M. A bacteriorhodopsin's proton pump model based on the FT-IR works is presented.

PDF Article

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.