Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 48,
  • Issue 2,
  • pp. 209-213
  • (1994)

Influence of the Nitrobenzene Extraction Technique on the Protein Molecule Conformation

Not Accessible

Your library or personal account may give you access

Abstract

The Raman spectra of lysozyme, ribonuclease, and BSA (bovine serum albumin) in aqueous solution extracted by nitrobenzene and the ultra-violet visible absorption spectra of BSA in aqueous solution have been studied. It has been proven that the structure of all the characteristic Raman and UV bands of BAS protein in aqueous solution is due to α-helical and random-coil conformations. The tyrosine residue is "exposed", and the conformation of the carbon atoms in the disulfide bridge <i>c-c-s-s-c-c</i> is <i>trans-gauche-gauche.</i> The influence of adding nitrobenzene to lysozyme, ribonuclease, and BSA aqueous solution has been studied. The study indicated that nitrobenzene treatment of protein aqueous solution is an efficient means for obtaining better Raman spectra.

PDF Article
More Like This
Conformational analysis of proteins with a dual polarisation silicon microring

J-W. Hoste, S. Werquin, T. Claes, and P. Bienstman
Opt. Express 22(3) 2807-2820 (2014)

Three-dimensional protein shape rendering in magnetized solution with Lambert—Beer law

HongYan Gu and WeiShan Chang
Appl. Opt. 51(20) 4827-4832 (2012)

Ge on Si waveguide mid-infrared absorption spectroscopy of proteins and their aggregates

Vinita Mittal, George Devitt, Milos Nedeljkovic, Lewis G. Carpenter, Harold M. H. Chong, James S. Wilkinson, Sumeet Mahajan, and Goran Z. Mashanovich
Biomed. Opt. Express 11(8) 4714-4722 (2020)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved