Abstract

Electrophoresis is an important separation method for proteins and nucleic acids. Electrophoresis enables separation of polyionic macromolecules by molecular weight, shape, secondary structure, and electric charge. For proteins, 5–20% polyacrylamide gels are almost universally used. The gels function both as anticonvective and as sieving media. The sieving properties are conveniently varied by varying gel composition. Because polyacrylamide gels absorb in the ultraviolet, direct absorbance measurements are rarely used. Instead, proteins or nucleic acids are detected and quantified by staining them with dye or colloidal metals.

Accommodating brightness and exposure levels in densitometry of stained polyacrylamide electrophoresis gels

Han Yen Tan, Tuck Wah Ng, and Oi Wah Liew
Appl. Opt. 49(9) 1623-1629 (2010)

Fiber based optical tweezers for simultaneous in situ force exertion and measurements in a 3D polyacrylamide gel compartment

Chaoyang Ti, Gawain M Thomas, Yundong Ren, Rui Zhang, Qi Wen, and Yuxiang Liu
Biomed. Opt. Express 6(7) 2325-2336 (2015)

Laser behavior and photostability characteristics of organic dye doped silicate gel materials

Edward T. Knobbe, Bruce Dunn, Peter D. Fuqua, and Fumito Nishida
Appl. Opt. 29(18) 2729-2733 (1990)

Characterization of thermo-optic coefficient and material loss of high refractive index silica sol-gel films in the visible and near-IR

Brian A. Rose, Ashley J. Maker, and Andrea M. Armani
Opt. Mater. Express 2(5) 671-681 (2012)

Athermal silicon optical add-drop multiplexers based on thermo-optic coefficient tuning of sol-gel material

Soha Namnabat, Kyung-Jo Kim, Adam Jones, Roland Himmelhuber, Christopher T. DeRose, Douglas C. Trotter, Andrew L. Starbuck, Andrew Pomerene, Anthony L. Lentine, and Robert A. Norwood
Opt. Express 25(18) 21471-21482 (2017)