Abstract

Fourier self-deconvolution (FSD) has been employed to enhance the resolution of the infrared spectra of proteins in the solid state and in D₂O solution. The feasibility of using diffuse reflectance spectrometry for measuring the infrared spectra of solid proteins has been demonstrated. FSD permits inherently broad absorption bands to be resolved into distinct peaks which can be associated with specific protein secondary structures. Because the areas of the resolved peaks are the same as the areas of the previously unidentifiable components, this new method should enable quantitative estimates of the proportion of each conformation in a protein to be calculated.

Characterizing amyloid protein conformation with a multi-resonant metasurface based biosensor in the infrared window

Chen Chen, Kaifei Wagn, and Xun Hou
Opt. Express 32(4) 5287-5300 (2024)

Noise in Fourier self-deconvolution

Jyrki K. Kauppinen, Douglas J. Moffatt, David G. Cameron, and Henry H. Mantsch
Appl. Opt. 20(10) 1866-1879 (1981)

High-resolution Fourier-transform cavity-enhanced absorption spectroscopy in the near-infrared using an incoherent broad-band light source

Johannes Orphal and Albert A. Ruth
Opt. Express 16(23) 19232-19243 (2008)

High-Resolution Fourier Transform Spectroscopy in the Far-Infrared

P. L. Richards
J. Opt. Soc. Am. 54(12) 1474-1484 (1964)

Protein conformation characterization via a silicon resonator-based optical sensor based on the combination of wavelength interrogation and dual polarization detection

Chen Chen, Kaifei Wang, and Xun Hou
Opt. Express 30(25) 44472-44486 (2022)