Abstract

Rapid scanning constant energy synchronous fluorescence spectrometry (RSCESFS) is evaluated for flow cell design, scan speed, and constant energy interval choice. Analytical figures of merit for several polycyclic aromatic hydrocarbons (PAHs) are given for several experimental conditions, for several flow cells, and for both RSCESFS and conventional (nonscanning) fluorescence spectrometry. With RSCESFS, absolute detection limits as low as 700 fg, linear dynamic ranges as great as five orders of magnitude and the time to record a complete 350 nm spectrum as short as 17 s have been obtained.

Rapid Scanning Interference Spectrometry

L. Mertz
J. Opt. Soc. Am. 50(3) AD3_6-AD3_6 (1960)

Scanning flow cytometer modified to distinguish phytoplankton cells from their effective size, effective refractive index, depolarization, and fluorescence

Luca Fiorani, Valeri P. Maltsev, Vyacheslav M. Nekrasov, Antonio Palucci, Konstantin A. Semyanov, and Valeria Spizzichino
Appl. Opt. 47(24) 4405-4412 (2008)

Rapid-scan coherent 2D fluorescence spectroscopy

Simon Draeger, Sebastian Roeding, and Tobias Brixner
Opt. Express 25(4) 3259-3267 (2017)

Beam-splitter and cell-window errors in fluorescence spectrometry

Klaus D. Mielenz
Appl. Opt. 18(24) 4134-4137 (1979)

Semiconductor laser-induced fluorescence detection in picoliter volume flow cells

Anders P. Larson, Henrik Ahlberg, and Staffan Folestad
Appl. Opt. 32(6) 794-805 (1993)