October 2020
Spotlight Summary by Dario Polli
Simultaneous stimulated Raman gain and loss detection (SRGAL)
Can you trust the images collected using coherent-Raman vibrational microscopy? In this paper by S. Heuke and co-workers, you will find not only the answer to this question, but also an innovative procedure to compensate for many artifacts that can affect the Stimulated Raman Scattering (SRS) technique, thus indeed allowing us to trust the microscopy images. SRS is a very powerful technique in the life sciences, enabling the visualization of morphological details in cells and tissues on the sub-micrometer scale. Unlike fluorescence microscopy, often suffering from photo-toxicity and employing exogenous markers that can perturb the system under study, SRS is a label-free technique that probes the vibrational response of cells and tissues, reflecting their chemical structure and providing an endogenous and chemically specific signature that can be exploited for the identification of the molecular constituents. SRS has seen a tremendous increase of interest recently, as it is opening a new window for cell biology research and clinical diagnosis, offering intrinsic three-dimensional sectioning capability in mapping the distribution of chemical bonds in real time.
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Article Information
Simultaneous stimulated Raman gain and loss detection (SRGAL)
Sandro Heuke, Alberto Lombardini, Edlef Büttner, and Hervé Rigneault
Opt. Express 28(20) 29619-29630 (2020) View: Abstract | HTML | PDF