Abstract

Circular intensity differential scattering (CIDS) is based on the analysis of circular polarized light scattering and has been proven to be an interesting label-free microscopy technique sensitive to the chiral organization at the submicroscopic level. However, this approach averages the localized contrasts related to the sample polarimetric properties in the illumination volume. Additionally, the detection sensitivity suffers from the confinement of the mixture of structures, and it becomes an arduous task to discriminate the source of the signal. In this work, we show that a phasor map approach combined with CIDS microscopy has provided an intuitive view of the sample organization to recognize the presence of different molecular species in the illumination volume. The data represented in terms of polarization response mapped to a single point called a phasor also have the potential to pave the way for the analysis of large data sets. We validated this method by numerical simulations and compared the results with that of experimental data of optical devices of reference.

© 2021 Optical Society of America

Full Article  |  PDF Article
More Like This
ExCIDS: a combined approach coupling Expansion Microscopy (ExM) and Circular Intensity Differential Scattering (CIDS) for chromatin-DNA imaging

Riccardo Marongiu, Aymeric Le Gratiet, Luca Pesce, Paolo Bianchini, and Alberto Diaspro
OSA Continuum 3(7) 1770-1780 (2020)

Circular intensity differential scattering (CIDS) scanning microscopy to image chromatin-DNA nuclear organization

Aymeric Le Gratiet, Luca Pesce, Michele Oneto, Riccardo Marongiu, Giulia Zanini, Paolo Bianchini, and Alberto Diaspro
OSA Continuum 1(3) 1068-1078 (2018)

Circular intensity differential scattering of light to characterize the coronavirus particles

Muhammad Waseem Ashraf, Rajeev Ranjan, and Alberto Diaspro
J. Opt. Soc. Am. B 38(5) 1702-1709 (2021)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (13)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription