Abstract

Digital holographic microscopy (DHM) suffers from a limited refocus depth range due to the employed microscope objective. The relationship between the refocus depth range of the DHM system and its optical parameters is studied theoretically and experimentally, and a method is proposed that combines wavefront coding with bicubic interpolation and extrapolation iteration to extend the refocus depth range. For obtaining a uniform point spread function on the refocus plane, a propagator kernel in numerical reconstruction is multiplied by a numerical logarithmic phase mask, and a deviation of the refocus distance is made. To improve the resolution of the refocused image, the size of the hologram is doubly enlarged. This approach can extend the refocus depth range from 0.9 up to 1.5 mm. This work provides important guidance for high-resolution imaging with a large refocus depth range in DHM.

© 2020 Optical Society of America

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Supplementary Material (7)

NameDescription
» Data File 1       Relationship between the refocus depth range and magnification of the DHM system
» Data File 2       Relationship between the refocus depth range and working distance of the MO
» Data File 3       Relationship between the refocus depth range and pupillary aperture of the MO (size of the image sensor)
» Data File 4       Relationship between the refocus depth range and pixel pitch of the image sensor
» Data File 5       measured sharpness of Fig.11-Fig.13
» Data File 6       measured sharpness of Fig.18
» Visualization 1       a reconstruction result of PSF in Fig.5 with d = 0.8

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Figures (21)

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Equations (17)

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