Abstract

Probing depth and system aberrations have direct impacts on the spatial resolution of stimulated emission depletion (STED) microscopes. Based on the vectorial diffraction theory, the influence of coma and astigmatism on the focal patterns of STED microscopes in probing stratified mediums with discontinuous refractive indices (e.g., glass cover slip, solution, and biological samples, etc.) have been illustrated in detail. The spatial resolution of the STED system has been discussed by analyzing the full width at half-maximum size of the fluorescence spots. It is found that, while probing in stratified media with discontinuous refractive indices, the spatial resolution of a STED microscope can be very sensitive to the existence of aberrations, e.g., coma and astigmatism, at different probing depths, as a result of mismatched axial positions of the excitation and depletion patterns. The spatial resolution of STED can be degraded up to 1.87- and 1.95-fold compared to that without aberrations. Therefore, a careful evaluation of the influence of aberration and discontinuous refractive indices should be taken into account when applying a STED microscope to realize super-resolution images.

© 2019 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Two-photon excitation STED microscopy by utilizing transmissive liquid crystal devices

Kohei Otomo, Terumasa Hibi, Yuichi Kozawa, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Shunichi Sato, and Tomomi Nemoto
Opt. Express 22(23) 28215-28221 (2014)

Effects of primary aberrations on the fluorescence depletion patterns of STED microscopy

Suhui Deng, Li Liu, Ya Cheng, Ruxin Li, and Zhizhan Xu
Opt. Express 18(2) 1657-1666 (2010)

Effects of aberrations on effective point spread function in STED microscopy

Yanghui Li, Hui Zhou, Xiaoyu Liu, Yuxue Li, and Le Wang
Appl. Opt. 57(15) 4164-4170 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (11)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription