Abstract

In this paper, we present a phase correlation imaging technique employing quantitative phase microscopy to study the migration properties of living cells. The phase correlation map relies on time-lapse quantitative phase stacks, which are achieved by single-shot quantitative phase imaging based on the transport of intensity equation. The phase correlation imaging maps can reveal tiny fluctuations in cellular optical path length and dynamic subdomains in living cells. We find that the morphology dynamics and intracellular mass transport rates are significantly different as cells exhibit different levels of migratory capabilities.

© 2019 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Quantitative phase microscopy for cellular dynamics based on transport of intensity equation

Ying Li, Jianglei Di, Chaojie Ma, Jiwei Zhang, Jinzhan Zhong, Kaiqiang Wang, Teli Xi, and Jianlin Zhao
Opt. Express 26(1) 586-593 (2018)

Dispersion-relation phase spectroscopy of intracellular transport

Ru Wang, Zhuo Wang, Larry Millet, Martha U. Gillette, A. J. Levine, and Gabriel Popescu
Opt. Express 19(21) 20571-20579 (2011)

Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport

Charles-Edouard Leroux, Fabien Bertillot, Olivier Thouvenin, and Albert-Claude Boccara
Biomed. Opt. Express 7(11) 4501-4513 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription