Abstract

The intracellular dynamics of onion epidermal cells during the dehydration process is observed by holographic microscopy. Both the nucleus and cytoplasm are accurately revealed by quantitative phase imaging while dehydration takes place. Indeed, we notice that the contrast of phase images increases with the decrease in cellular water content. We foresee that such a dehydrating process can be effective for improving phase contrast, thus permitting better imaging of plant cells with the scope of learning more about cellular dynamics and related phenomena. Exploiting this concept, we observe intracellular cytoplasmic circulation and transport of biological material.

© 2019 Optical Society of America

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Supplementary Material (2)

NameDescription
» Visualization 1       Visualization 1 shows a DH phase contrast time lapse of various onion epidermal cells undergoing dehydration.
» Visualization 2       Visualization 2 shows a DH phase contrast time lapse of one single onion epidermal cell undergoing dehydration and reveals cytoplasm circulation with intracellular transport of organelles.

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