Sensitive determination of DNA based on phosphate-dye interaction using photothermal lens technique

Nader Shokoufi; Behnaz Abbasgholi Nejad Asbaghi; Shafigh Nouri Hajibaba

doi:10.1364/AO.58.003074

Applied Optics
Vol. 58,
Issue 12,
pp. 3074-3082
(2019)
•https://doi.org/10.1364/AO.58.003074

Sensitive determination of DNA based on phosphate-dye interaction using photothermal lens technique

Nader Shokoufi, Behnaz Abbasgholi Nejad Asbaghi, and Shafigh Nouri Hajibaba

Not Accessible

Your library or personal account may give you access

Get PDF
Email
Share
Get Citation
Copy Citation Text
Nader Shokoufi, Behnaz Abbasgholi Nejad Asbaghi, and Shafigh Nouri Hajibaba, "Sensitive determination of DNA based on phosphate-dye interaction using photothermal lens technique," Appl. Opt. 58, 3074-3082 (2019)

Export Citation
- BibTex
- Endnote (RIS)
- HTML
- Plain Text
Citation alert
Save article

Check for updates

Abstract

Photothermal lens spectrometry is a powerful optical detection technique that can be used to investigate biomolecules. In this work, for the first time to our knowledge, photothermal lens spectrometry was used for determination of nanomolar concentrations of three distinct deoxyribonucleic acid (DNA) strands using methylene blue as a labeling dye. Methylene blue interacts with phosphate groups of the DNA in lower DNA concentrations. It was observed that phosphate–methylene blue interaction had no obvious effect on methylene blue absorption and fluorescence spectra, but the photothermal lens spectrometry signal of methylene blue increased with DNA concentration. For this purpose, to evaluate the performance of the presented method, herring sperm DNA, Escherichia coli bacteria DNA, and partial 16S rRNA genes were examined. Under optimum conditions, photothermal lens spectrometry intensity of methylene blue increased linearly with DNA concentration when herring sperm DNA, Escherichia coli DNA, and 16S rRNA gene concentrations increased in the ranges of 0.1–250, 1–700, and $1 - 800 nmol L^{- 1}$ , respectively. The corresponding detection limits were found to be 0.07, 0.71, and $0.56 nmol L^{- 1}$ , respectively, and relative standard deviations for $50 nmol L^{- 1}$ of the tested samples were 2.59%, 4.95%, and 4.57%, respectively.

Full Article | PDF Article

More Like This

Quantitative analysis of double-stranded DNA amplified by a polymerase chain reaction employing surface-enhanced Raman spectroscopy

X. Dou, T. Takama, Y. Yamaguchi, K. Hirai, H. Yamamoto, S. Doi, and Y. Ozaki
Appl. Opt. 37(4) 759-763 (1998)

Convective-diffusion-based fluorescence correlation spectroscopy for detection of a trace amount of E. coli in water

De-Kui Qing, M. Pinar Mengüç, Fred A. Payne, and Mary-Grace C. Danao
Appl. Opt. 42(16) 2987-2994 (2003)

Determination of linear and nonlinear absorption of metallic colloids using photothermal lens spectrometry

A. Marcano O., F. Delima, Y. Markushin, and N. Melikechi
J. Opt. Soc. Am. B 28(2) 281-287 (2011)

Previous Article Next Article

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (4)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

	Analytical Feature
Parameter	hs DNA	E. Coli DNA	16S rRNA Gene
Linear range ( $nmol L^{- 1}$ )	0.1–250	1–700	1–800
Limit of detection^a ( $nmol L^{- 1}$ ) ( $n = 5$ )	0.068	0.712	0.558
Repeatability (R.S.D) % ( $n = 5$ )	2.59	4.95	4.57
Calibration curve equation	$y = 0.004 c + 1.4401$	$y = 0.0009 c + 0.156$	$y = 0.0014 c + 0.2899$
$R^{2}$	0.9966	0.9910	0.9890

Substances	Tolerable Molar Excess
Adenine	10
Thymine	10
Cytosine	10
Guanine	10
Bovine serum albumin	2^a
${Ca}^{2 +}$	5
${Mg}^{2 +}$	0.5
${Zn}^{2 +}$	1
${PO}_{4}^{3 -}$	15

DNA Samples	Added ( $nmol L^{- 1}$ )	Found ( $nmol L^{- 1}$ )	Recovery (%)	RSD ( $n = 5$ )
hs DNA	50	51.3	102.6	3.2
E. coli DNA	50	51.9	103.8	5.7
16S rRNA gene	50	52.1	104.2	6.2

Detection Method	Kind of DNA	Linearity Range ( $μmol L^{- 1}$ )	Detection Limit ( $μmol L^{- 1}$ )	Sample Volume	RSD %	Ref.
Fluorescence	ct DNA^a	13.6–1280	2.28		4.22	[54]
Fluorescence	ct DNA	0.28–11.0	0.11	10 ml	–	[52]
Fluorescence	hs DNA	0.28–8.25	0.04	10 ml	–
Carbon dots-based fluorescence turn-on	ct DNA	3–80	1	0.5	2.8	[55]
Carbon dots-based fluorescence turn-on	hs DNA	10-4-0.25	$0.68 \times 10^{- 4}$	0.5 ml	2.59
Photothermal lens spectrometry based on the interaction of methylene blue with DNA	E. coli DNA	10-3-0.7	$0.712 \times 10^{- 3}$	0.5 ml	4.95	This work
	16S rRNA gene	10-3-0.8	$0.558 \times 10^{- 3}$	0.5 ml	4.57

	Analytical Feature
Parameter	hs DNA	E. Coli DNA	16S rRNA Gene
Linear range ( $nmol L^{- 1}$ )	0.1–250	1–700	1–800
Limit of detection^a ( $nmol L^{- 1}$ ) ( $n = 5$ )	0.068	0.712	0.558
Repeatability (R.S.D) % ( $n = 5$ )	2.59	4.95	4.57
Calibration curve equation	$y = 0.004 c + 1.4401$	$y = 0.0009 c + 0.156$	$y = 0.0014 c + 0.2899$
$R^{2}$	0.9966	0.9910	0.9890

Abstract

Cited By

Figures (10)

Tables (4)

Equations (1)

Applied Optics