Abstract

A numerical simulation tool is reported for nanometer thin and inhomogeneous immobilized protein films on gold in aqueous solution. It allows for the first time, to the best of our knowledge, the simultaneous assessment of refractive index, film thickness, and surface coverage. The model relies on and combines the convective diffusion equation, the Langmuir adsorption isotherm, and the Helmholtz equation, with appropriate boundary conditions. These three differential equations were jointly solved using a multiphysics software. The physical film parameters were extracted employing an optimization procedure for immobilized bovine serum albumin, hemoglobin, and neutravidin films. The relatively good agreement between the extracted values for the refractive index, film thickness, and surface coverage and the corresponding values reported in the open literature show the correctness of the proposed methodology.

© 2018 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Biofunctionalized tilted Fiber Bragg Gratings for label-free immunosensing

Séverine Maguis, Guillaume Laffont, Pierre Ferdinand, Benjamin Carbonnier, Khémara Kham, Tahar Mekhalif, and Marie-Claude Millot
Opt. Express 16(23) 19049-19062 (2008)

Investigation for terminal reflection optical fiber SPR glucose sensor and glucose sensitive membrane with immobilized GODs

Yinquan Yuan, Xi Yang, Dejing Gong, Fang Liu, Wenbin Hu, Weiquan Cai, Jun Huang, and Minghong Yang
Opt. Express 25(4) 3884-3898 (2017)

Ellipsometry study on gold-nanoparticle-coated gold thin film for biosensing application

Rakesh Singh Moirangthem, Yia-Chung Chang, and Pei-Kuen Wei
Biomed. Opt. Express 2(9) 2569-2576 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (10)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (24)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription