Multispectral phase imaging based on acousto-optic filtration of interfering light beams [Invited]

Alexander Machikhin; Ludmila Burmak; Olga Polschikova; Alina Ramazanova; Vitold Pozhar; Sergei Boritko

doi:10.1364/AO.57.000C64

Applied Optics
Vol. 57,
Issue 10,
pp. C64-C69
(2018)
•https://doi.org/10.1364/AO.57.000C64

Multispectral phase imaging based on acousto-optic filtration of interfering light beams [Invited]

Alexander Machikhin, Ludmila Burmak, Olga Polschikova, Alina Ramazanova, Vitold Pozhar, and Sergei Boritko

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Alexander Machikhin, Ludmila Burmak, Olga Polschikova, Alina Ramazanova, Vitold Pozhar, and Sergei Boritko, "Multispectral phase imaging based on acousto-optic filtration of interfering light beams [Invited]," Appl. Opt. 57, C64-C69 (2018)

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Abstract

Acousto-optic spectral selection of light is an effective technique for interference imaging at multiple wavelengths. In this paper, we show that, depending on the location of the acousto-optical tunable filter relative to the interferometer, it is possible to enhance important characteristics of the whole system: spectral contrast, insensitivity to ambient light, performance stability, and measurement accuracy. We analyze theoretically and compare experimentally a quantitative phase imaging system based on a Mach–Zehnder interferometer with one and two acousto-optical filters located in the illumination or/and in the output channels. Visibility of the interference patterns and noise root mean square in the calculated phase maps are estimated for all cases at room temperature. It is shown that acousto-optic filtration of interfering light beams in the output channel ensures better contrast of the interference pattern and, therefore, provides better stability and higher precision of the phase measurements.

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	1st Sidelobe ( $α$ )		Out-of-band Ratio ( $β$ )
	$T_{0} ≪ 1$	$T_{0} = 1$	$T_{0} ≪ 1$	$T_{0} = 1$
Single AOTF	4.7%	11.6%	9.7%	21%
Double AOTF	0.2%	1.4%	0.3%	2%

Feature	Optical Scheme
Feature	$AO + I$	$AO + I + AO$	$I + AO$
Spectral contrast $(1 - β) / β$	4	50	4
Depolarization suppression	−	+	+
Insensitivity to ambient light	−	+	+
Applicability to fluorescence and Raman imaging	−	+	–
Transmission ^a	$\sim 50 %$	$≲ 50 %$ ^b	$\sim 50 %$

	1st Sidelobe ( $α$ )		Out-of-band Ratio ( $β$ )
	$T_{0} ≪ 1$	$T_{0} = 1$	$T_{0} ≪ 1$	$T_{0} = 1$
Single AOTF	4.7%	11.6%	9.7%	21%
Double AOTF	0.2%	1.4%	0.3%	2%

Feature	Optical Scheme
Feature	$AO + I$	$AO + I + AO$	$I + AO$
Spectral contrast $(1 - β) / β$	4	50	4
Depolarization suppression	−	+	+
Insensitivity to ambient light	−	+	+
Applicability to fluorescence and Raman imaging	−	+	–
Transmission ^a	$\sim 50 %$	$≲ 50 %$ ^b	$\sim 50 %$

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Figures (7)

Tables (2)

Equations (4)

Applied Optics