Abstract

The 2×2 ray tracing matrix (RTM) method is employed for the description of optical aberrations caused by the refractive index mismatch (RIM) in fluorescent confocal polarization microscopy. We predict and experimentally confirm that due to the RIM a liquid crystal layer with highly non-uniform director distribution appears to be imaged as a layer with non-uniform thickness, which shows up in the roughness of the rear surface. For the off-axial focusing of the probing beam in a droplet dispersed in an immiscible liquid, we have developed an extended method still keeping the 2×2 dimensionality of the RTM.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens

Cees J. de Grauw, Jurrien M. Vroom, Hans T. M van der Voort, and Hans C. Gerritsen
Appl. Opt. 38(28) 5995-6003 (1999)

Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy

Alberto Diaspro, Federico Federici, and Mauro Robello
Appl. Opt. 41(4) 685-690 (2002)

Depth profiles in confocal optical microscopy: a simulation approach based on the second Rayleigh-Sommerfeld diffraction integral

Rosario Esposito, Giuseppe Scherillo, Marianna Pannico, Pellegrino Musto, Sergio De Nicola, and Giuseppe Mensitieri
Opt. Express 24(12) 12565-12576 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (38)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription