Abstract

The fundamental limit set by the wavelength of light can be overcome using methods of superresolution localization microscopy. These methods require labeling of the sample with fluorescent molecules and are time consuming as repeated cycles of activation and photobleaching of the sample are required. Alternatively, we propose a simplified approach that is free from direct labeling with fluorescence molecules and does not require the repeated cycles of activation and photobleaching. The method uses fluorescently labeled gold nanoparticles in an aqueous solution that are distributed on top of the sample. The nanoparticles move in random Brownian motion and obscure different areas of the sample, while the scene is being imaged sequentially. By conducting the proper postprocessing, a superresolution image can be generated. The method is validated both by numerical simulations as well as by experimental data.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Superresolved labeling nanoscopy based on temporally flickering nanoparticles and the K-factor image deshadowing

Tali Ilovitsh, Yossef Danan, Asaf Ilovitsh, Amihai Meiri, Rinat Meir, and Zeev Zalevsky
Biomed. Opt. Express 6(4) 1262-1272 (2015)

Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles

F. Verpillat, F. Joud, P. Desbiolles, and M. Gross
Opt. Express 19(27) 26044-26055 (2011)

Phase stretch transform for super-resolution localization microscopy

Tali Ilovitsh, Bahram Jalali, Mohammad H. Asghari, and Zeev Zalevsky
Biomed. Opt. Express 7(10) 4198-4209 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (1)

NameDescription
» Visualization 1: MP4 (7419 KB)      Construction of the super resolution image

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription