Abstract

We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multiangle acquisitions on 3D cell cultures embedded in extracellular matrices. Lens-free microscopy acquisitions present some inherent issues such as the lack of phase information on the sensor plane and a limited angular coverage. We developed and compared three different algorithms based on the Fourier diffraction theorem to obtain fully 3D reconstructions. These algorithms present an increasing complexity associated with a better reconstruction quality. Two of them are based on a regularized inverse problem approach. To compare the reconstruction methods in terms of artefact reduction, signal-to-noise ratio, and computation time, we tested them on two experimental datasets: an endothelial cell culture and a prostate cell culture grown in a 3D extracellular matrix with large reconstructed volumes up to 5  mm3 with a resolution sufficient to resolve isolated single cells. The lens-free reconstructions compare well with standard microscopy.

© 2017 Optical Society of America

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Supplementary Material (6)

NameDescription
» Visualization 1: AVI (1102 KB)      Visualization_1_HUVEC_3D_view
» Visualization 2: AVI (2021 KB)      Visualization_2_RWPE1_3D_view
» Visualization 3: AVI (4036 KB)      Visualization_3_RWPE1_3D_view
» Visualization 4: TIF (2512 KB)      Visualization_4_comparison_microscope_average
» Visualization 5: AVI (5544 KB)      Visualization_5_comparison_microscope_z-stack
» Visualization 6: TIF (11202 KB)      Visualization_6_comparison_microscope_z-stack

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