Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Nanoscale three-dimensional single particle tracking by light-sheet-based double-helix point spread function microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The double-helix point spread function (DH-PSF) microscopy has become an essential tool for nanoscale three-dimensional (3D) localization and tracking of single molecules in living cells. However, its localization precision is limited by fluorescent contrast in thick samples because the signal-to-noise ratio of the system is low due to the inherent low transfer function efficiency and background fluorescence. Here we combine DH-PSF microscopy with light-sheet illumination to eliminate out-of-focus background fluorescence for high-precision 3D single particle tracking. To demonstrate the capability of the method, we obtain the single fluorescent bead image with light-sheet illumination, with three-dimensional localization accuracy better than that of epi-illumination. We also show that the single fluorescent beads in agarose solution can be tracked, which demonstrates the possibility of our method for the study of dynamic processes in complex biological specimens.

© 2016 Optical Society of America

Full Article  |  PDF Article
More Like This
Three-dimensional parallel particle manipulation and tracking by integrating holographic optical tweezers and engineered point spread functions

Donald B. Conkey, Rahul P. Trivedi, Sri Rama Prasanna Pavani, Ivan I. Smalyukh, and Rafael Piestun
Opt. Express 19(5) 3835-3842 (2011)

Three-dimensional diffusion coefficient measurement by a large depth-of-field rotating point spread function

Famin Wang, Hangfeng Li, Lin Ji, Mengyuan Zhao, Xin Miu, Yunhai Zhang, Wei Huang, and Tongda Wei
Appl. Opt. 60(35) 10766-10771 (2021)

Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system

Sri Rama Prasanna Pavani and Rafael Piestun
Opt. Express 16(26) 22048-22057 (2008)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved