Abstract

The double-helix point spread function (DH-PSF) microscopy has become an essential tool for nanoscale three-dimensional (3D) localization and tracking of single molecules in living cells. However, its localization precision is limited by fluorescent contrast in thick samples because the signal-to-noise ratio of the system is low due to the inherent low transfer function efficiency and background fluorescence. Here we combine DH-PSF microscopy with light-sheet illumination to eliminate out-of-focus background fluorescence for high-precision 3D single particle tracking. To demonstrate the capability of the method, we obtain the single fluorescent bead image with light-sheet illumination, with three-dimensional localization accuracy better than that of epi-illumination. We also show that the single fluorescent beads in agarose solution can be tracked, which demonstrates the possibility of our method for the study of dynamic processes in complex biological specimens.

© 2016 Optical Society of America

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References

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    [Crossref]
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    [Crossref]
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2014 (3)

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

2013 (1)

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

2012 (1)

J. H. Spille, T. Kaminski, H. P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 220, 19697–19707 (2012).
[Crossref]

2011 (2)

2010 (6)

M. F. Juette and J. Bewersdorf, “Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution,” Nano Lett. 10, 4657–4663 (2010).
[Crossref]

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

S. J. Lord, H.-l. D. Lee, and W. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem. 82, 2192–2203 (2010).
[Crossref]

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

2009 (2)

J. Huisken and D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[Crossref]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

2008 (6)

M. Tokunaga, N. Imamoto, and K. Sakata-Sogawa, “Highly inclined thin illumination enables clear single-molecule imaging in cells,” Nat. Methods 5, 159–161 (2008).
[Crossref]

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16, 3484–3489 (2008).
[Crossref]

J. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16, 7142–7152 (2008).
[Crossref]

2007 (4)

J. Huisken and D. Y. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32, 2608–2610 (2007).
[Crossref]

K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum. 78, 023705 (2007).
[Crossref]

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90, 053902 (2007).
[Crossref]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

2004 (2)

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J. 86, 1185–1200 (2004).
[Crossref]

2002 (2)

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82, 2775–2783 (2002).
[Crossref]

E. Fuchs, J. Jaffe, R. Long, and F. Azam, “Thin laser light sheet microscope for microbial oceanography,” Opt. Express 10, 145–154 (2002).
[Crossref]

2000 (1)

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

1994 (1)

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Azam, F.

Backlund, M. P.

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

Badieirostami, M.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

Bene, F. D.

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

Bennett, B. T.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Bewersdorf, J.

M. F. Juette and J. Bewersdorf, “Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution,” Nano Lett. 10, 4657–4663 (2010).
[Crossref]

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Biteen, J. S.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Brown, P. O.

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

Cang, H.

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Cao, B.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

Casolari, J. M.

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

Chen, D.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

Fiedler, C.

Fuchs, E.

Funatsu, T.

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Gould, T. J.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Greger, K.

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum. 78, 023705 (2007).
[Crossref]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

Grover, G.

Harada, Y.

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Hess, S. T.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Holtzer, L.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90, 053902 (2007).
[Crossref]

Hu, Y. S.

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Huisken, J.

J. Huisken and D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[Crossref]

J. Huisken and D. Y. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32, 2608–2610 (2007).
[Crossref]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

Imamoto, N.

M. Tokunaga, N. Imamoto, and K. Sakata-Sogawa, “Highly inclined thin illumination enables clear single-molecule imaging in cells,” Nat. Methods 5, 159–161 (2008).
[Crossref]

Jaffe, J.

Joyner, R.

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

Juette, M. F.

M. F. Juette and J. Bewersdorf, “Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution,” Nano Lett. 10, 4657–4663 (2010).
[Crossref]

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Kaminski, T.

J. H. Spille, T. Kaminski, H. P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 220, 19697–19707 (2012).
[Crossref]

Keller, P. J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

Königshoven, H. P.

J. H. Spille, T. Kaminski, H. P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 220, 19697–19707 (2012).
[Crossref]

Kržic, U.

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

Kubitscheck, U.

J. H. Spille, T. Kaminski, H. P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 220, 19697–19707 (2012).
[Crossref]

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

J. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16, 7142–7152 (2008).
[Crossref]

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

Küchmann, O.

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

Kues, T.

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

Larson, D. R.

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82, 2775–2783 (2002).
[Crossref]

Lee, H.-l. D.

S. J. Lord, H.-l. D. Lee, and W. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem. 82, 2192–2203 (2010).
[Crossref]

Lessard, M. D.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Lew, M. D.

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

Li, H.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

Li, Y.

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Lin, D.

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

Lin, N.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Liu, X.

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

Long, R.

Lord, S. J.

S. J. Lord, H.-l. D. Lee, and W. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem. 82, 2192–2203 (2010).
[Crossref]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Ma, W.

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

Marcello, M.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

Meckel, T.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90, 053902 (2007).
[Crossref]

Mlodzianoski, M. J.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Moerner, W.

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

S. J. Lord, H.-l. D. Lee, and W. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem. 82, 2192–2203 (2010).
[Crossref]

Moerner, W. E.

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Nagpure, B. S.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

Niu, H.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

Ober, R. J.

R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J. 86, 1185–1200 (2004).
[Crossref]

Pampaloni, F.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

Pavani, S. R. P.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16, 3484–3489 (2008).
[Crossref]

Peters, R.

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

Piestun, R.

G. Grover, S. Quirin, C. Fiedler, and R. Piestun, “Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging,” Biomed. Opt. Express 2, 3010–3012 (2011).
[Crossref]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16, 3484–3489 (2008).
[Crossref]

Quirin, S.

Ram, S.

R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J. 86, 1185–1200 (2004).
[Crossref]

Reynaud, E. G.

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

Ritter, J.

Ritter, J. G.

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

Sakata-Sogawa, K.

M. Tokunaga, N. Imamoto, and K. Sakata-Sogawa, “Highly inclined thin illumination enables clear single-molecule imaging in cells,” Nat. Methods 5, 159–161 (2008).
[Crossref]

Santi, P. A.

P. A. Santi, “Light sheet fluorescence microscopy a review,” J. Histochem. Cytochem. 59, 129–138 (2011).
[Crossref]

Satito, K.

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

Schmidt, T.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90, 053902 (2007).
[Crossref]

Siebrasse, J. P.

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

J. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16, 7142–7152 (2008).
[Crossref]

Spille, J. H.

J. H. Spille, T. Kaminski, H. P. Königshoven, and U. Kubitscheck, “Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue,” Opt. Express 220, 19697–19707 (2012).
[Crossref]

Stainier, D. Y.

Stainier, D. Y. R.

J. Huisken and D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[Crossref]

Stelzer, E. H.

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

Stelzer, E. H. K.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum. 78, 023705 (2007).
[Crossref]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

Sun, Y.

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

Swoger, J.

K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum. 78, 023705 (2007).
[Crossref]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

Thompson, M. A.

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Thompson, R. E.

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82, 2775–2783 (2002).
[Crossref]

Tokunaga, M.

M. Tokunaga, N. Imamoto, and K. Sakata-Sogawa, “Highly inclined thin illumination enables clear single-molecule imaging in cells,” Nat. Methods 5, 159–161 (2008).
[Crossref]

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Twieg, R. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

Veenendaal, A.

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

Veith, R.

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

J. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16, 7142–7152 (2008).
[Crossref]

Verveer, P. J.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

Ward, E. S.

R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J. 86, 1185–1200 (2004).
[Crossref]

Watters, R.

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Webb, W. W.

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82, 2775–2783 (2002).
[Crossref]

Weis, K.

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

Wu, Y.

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

Yanagida, T.

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Yu, B.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

Yu, J.

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

Zimmerley, M.

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Acta Phys. Sinica (1)

H. Li, B. Yu, D. Chen, and H. Niu, “Design and experimental demonstration of high-efficiency double-helix point spread function phase plate,” Acta Phys. Sinica 62, 124201 (2013).

Anal. Chem. (1)

S. J. Lord, H.-l. D. Lee, and W. Moerner, “Single-molecule spectroscopy and imaging of biomolecules in living cells,” Anal. Chem. 82, 2192–2203 (2010).
[Crossref]

Appl. Phys. Lett. (2)

M. Badieirostami, M. D. Lew, M. A. Thompson, and W. Moerner, “Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane,” Appl. Phys. Lett. 97, 161103 (2010).
[Crossref]

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett. 90, 053902 (2007).
[Crossref]

Biomed. Opt. Express (1)

Biophys. J. (3)

U. Kubitscheck, O. Küchmann, T. Kues, and R. Peters, “Imaging and tracking of single GFP molecules in solution,” Biophys. J. 78, 2170–2179 (2000).
[Crossref]

R. E. Thompson, D. R. Larson, and W. W. Webb, “Precise nanometer localization analysis for individual fluorescent probes,” Biophys. J. 82, 2775–2783 (2002).
[Crossref]

R. J. Ober, S. Ram, and E. S. Ward, “Localization accuracy in single-molecule microscopy,” Biophys. J. 86, 1185–1200 (2004).
[Crossref]

Chem. Phys. Chem. (1)

Y. S. Hu, M. Zimmerley, Y. Li, R. Watters, and H. Cang, “Single-molecule super-resolution light-sheet microscopy,” Chem. Phys. Chem. 15, 577–586 (2014).

Development (1)

J. Huisken and D. Y. R. Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[Crossref]

HFSP J. (1)

E. G. Reynaud, U. Kržič, K. Greger, and E. H. Stelzer, “Light sheet-based fluorescence microscopy: More dimensions, more photons, and less photodamage,” HFSP J. 2, 266–275 (2008).
[Crossref]

J. Histochem. Cytochem. (1)

P. A. Santi, “Light sheet fluorescence microscopy a review,” J. Histochem. Cytochem. 59, 129–138 (2011).
[Crossref]

Mol. Biol. Cell (1)

M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope,” Mol. Biol. Cell 25, 3619–3629 (2014).
[Crossref]

Nano Lett. (2)

M. A. Thompson, M. D. Lew, M. Badieirostami, and W. E. Moerner, “Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function,” Nano Lett. 10, 211–218 (2010).
[Crossref]

M. F. Juette and J. Bewersdorf, “Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution,” Nano Lett. 10, 4657–4663 (2010).
[Crossref]

Nat. Methods (3)

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Methods 5, 527–529 (2008).
[Crossref]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4, 311–313 (2007).

M. Tokunaga, N. Imamoto, and K. Sakata-Sogawa, “Highly inclined thin illumination enables clear single-molecule imaging in cells,” Nat. Methods 5, 159–161 (2008).
[Crossref]

Nature (1)

T. Funatsu, Y. Harada, M. Tokunaga, K. Satito, and T. Yanagida, “Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution,” Nature 374, 555–559 (1994).
[Crossref]

Opt. Express (4)

Opt. Lett. (1)

PloS One (1)

J. G. Ritter, R. Veith, A. Veenendaal, J. P. Siebrasse, and U. Kubitscheck, “Light sheet microscopy for single molecule tracking in living tissue,” PloS One 5, e11639 (2010).
[Crossref]

Proc. Natl. Acad. Sci. USA (2)

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Lin, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 106, 2995–2999 (2009).
[Crossref]

M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, and W. Moerner, “Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA 107, 17864–17871 (2010).
[Crossref]

Proc. SPIE (1)

J. Yu, B. Cao, H. Li, B. Yu, D. Chen, and H. Niu, “Improved localization accuracy in double-helix point spread function super-resolution fluorescence microscopy using selective-plane illumination,” Proc. SPIE 9230, 92300N (2014).
[Crossref]

Rev. Sci. Instrum. (1)

K. Greger, J. Swoger, and E. H. K. Stelzer, “Basic building units and properties of a fluorescence single plane illumination microscope,” Rev. Sci. Instrum. 78, 023705 (2007).
[Crossref]

Science (2)

J. Huisken, J. Swoger, F. D. Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322, 1065–1069 (2008).
[Crossref]

Other (1)

D. Lin, W. Ma, X. Liu, Y. Sun, and Y. Wu, “Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution,” in Proceedings of IEEE Conference on Image and Signal Processing (IEEE, 2008), pp. 244–248.

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Figures (7)

Fig. 1.
Fig. 1.

Schematic of the DH-PSF microscopy using light-sheet illumination.

Fig. 2.
Fig. 2.

Design of PM. (a) The image shows the simulation result of PM. (b) The actual fabricated PM using the microfabrication photoetching method.

Fig. 3.
Fig. 3.

Dimensions of the light sheet. (a) The axial extension of the light sheet. (b) FWHM of the axial width. (c) FWHM of the light sheet along the illumination axis.

Fig. 4.
Fig. 4.

Contrast improvement in LSFM versus epi-illumination microscopy. Example DH-PSF images showing the fluorescence beads upon LSFM (a) and epi-illumination microscopy (b). (c) Fluorescent bead intensity profiles along the white line on image (a) and (b), indicating the contrast improvement.

Fig. 5.
Fig. 5.

Improvement of 3D localization accuracy by LSFM compared with epi-illumination microscopy. (a) Histogram of 2000 localizations of one single fluorescence bead in x, y, z. The smooth curve is a Gaussian fit in each case, which reflected 3D localization accuracy. The sample was imaged upon light-sheet illumination. (b) Using DH-PSF with epi-illumination for comparison.

Fig. 6.
Fig. 6.

Calibration curve of angle between two lobes with respect to the horizontal versus axial position measured with a piezo-controlled objective.

Fig. 7.
Fig. 7.

3D tracking and trajectory analysis of a single fluorescent bead. (a) Representative frames of the trajectory. (b) Gaussian distribution of relative displacements. (c) 3D trajectory. (d) Linear fitting of 3D-MSD.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

MSD(3)(nΔt)=i=1Nn[(xi+nxi)2+(yi+nyi)2+(zi+nzi)2]/(Nn),
MSD(3)(nΔt)=6D(3)·nΔt,
r=kT6πηD,

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