Abstract

The super-resolution optical fluctuation imaging (SOFI) technique enhances image spatial resolution by calculating the spatiotemporal cross-cumulants of independent stochastic intensity fluctuations of emitters. Ideally, SOFI eliminates any noise that is not correlated over time, but in practice, due to limited data lengths, the statistical uncertainty of cumulants will affect the continuities and homogeneities of SOFI images. Since the variance and signal-to-noise ratio (SNR) characterize cumulant statistical uncertainty, we determined theoretical expressions for these based on a single dataset. From a simulation of temporal fluctuations of blinking fluorescent emitters, we calculated the quantitative relation between the SNR of cumulants and multiple parameters of the blinking signal, such as the on-time ratio, acquisition frame to average blinking rate ratio, sequence length, and photon amplitude, which not only provides a physical interpretation for SOFI phenomena but also theoretical guidance to achieve optimal practical outcomes.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Comparison between SOFI and STORM

Stefan Geissbuehler, Claudio Dellagiacoma, and Theo Lasser
Biomed. Opt. Express 2(3) 408-420 (2011)

Super-resolution photoacoustic imaging via flow-induced absorption fluctuations

Thomas Chaigne, Bastien Arnal, Sergey Vilov, Emmanuel Bossy, and Ori Katz
Optica 4(11) 1397-1404 (2017)

Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Jörg Enderlein, and Shimon Weiss
Opt. Express 18(18) 18875-18885 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription