Abstract

The dynamic motions of various particles suspended in microscale flows are essential phenomena in the scientific and engineering fields. These motions can be precisely measured by using 3D quantitative flow visualization techniques. Moreover, most cells and particles in nature possess a nonspherical shape. Digital holographic microscopy (DHM) is employed to measure the 3D positional information of transparent ellipsoidal particles. Both in-plane and out-of-plane positional information are obtained by analyzing the distinctive light scattering from the microsized ellipsoidal particles. The performance of the 3D position measurement method is experimentally verified for ellipsoidal particles seeded in a planar surface and a microtube. This DHM technique exhibits promising potential in the dynamic analysis of ellipsoidal particles and cells suspended in various microscale fluid flows.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Precise measurement of orientations of transparent ellipsoidal particles through digital holographic microscopy

Hyeokjun Byeon, Taesik Go, and Sang Joon Lee
Opt. Express 24(1) 598-610 (2016)

Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy

Yong-Seok Choi and Sang-Joon Lee
Appl. Opt. 48(16) 2983-2990 (2009)

Digital holographic microscope for measuring three-dimensional particle distributions and motions

Jian Sheng, Edwin Malkiel, and Joseph Katz
Appl. Opt. 45(16) 3893-3901 (2006)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription