Abstract

For super-resolution microscopy methods based on single molecule stochastic switching and localization, to simultaneously improve the spatial–temporal resolution, it is necessary to maximize the number of photons that can be collected from single molecules per unit time. Here, we describe a novel approach to enhance the signal intensity (collected photons per second) from fluorescence probes by introducing a stimulated emission (SE) optical process. This process is based on the following two properties: first, with reasonable parameters, the photon emission rate can be significantly increased with SE; and second, the SE photons, which are spatially coherent with the stimulation beam, are more favorable for collection than fluorescence. Theoretical results have shown that signal intensity from a single fluorescent molecule can be greatly improved with SE. We therefore showed, using SE in combination with single molecule localization methodology, that fast imaging at a rate of 0.05 s per reconstructed image with lateral resolutions of 30nm can be obtained.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy

P. Zhang, P. M. Goodwin, and J. H. Werner
Opt. Express 22(10) 12398-12409 (2014)

Nonlinear structured illumination microscopy by surface plasmon enhanced stimulated emission depletion

Han Zhang, Ming Zhao, and Leilei Peng
Opt. Express 19(24) 24783-24794 (2011)

Isotropic 3D Nanoscopy based on single emitter switching

Claas v. Middendorff, Alexander Egner, Claudia Geisler, StefanW. Hell, and Andreas Schönle
Opt. Express 16(25) 20774-20788 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription