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Direct detection of aggregates in highly turbid colloidal suspensions of polystyrene nanoparticles

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Abstract

We demonstrate a total internal reflection-based method that detects, for the first time to the best of our knowledge, directly without any sample dilution or special sample preparation, the presence of aggregates in highly turbid aqueous suspensions of polystyrene nanospheres. Aggregation is induced by changing either the sample pH or ionic strength. The polystyrene mass density in our samples is two orders of magnitude higher than previously reported polystyrene aggregation studies. In cases when aggregates have formed but do not yet occupy a significant fraction of the sample volume, our sensor outperforms state of the art techniques such as dynamic light scattering in terms of sensitivity. Conversely, when the sample volume is dominated by aggregates, our sensor is not as effective.

© 2015 Optical Society of America

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