Abstract

Dual-mode super-resolution imaging system with two different super-resolution imaging methods, STED and FED, is presented. Electrical shutters controlled by the host computer are introduced to switch the two imaging modes. Principles of both methods are analyzed theoretically, and enhancements in the lateral resolution and SNR are demonstrated theoretically and experimentally. Results show that both imaging methods offered by the proposed system can break the diffraction barrier. Furthermore, the presented system provides a meaningful way to image fluorescent samples by a corresponding imaging mode according to the specific characteristics of samples analyzed for study. For samples that can endure high-power illumination, it is appropriate to use the STED mode to achieve a better resolution, while for samples that are vulnerable to high intensity, the FED method is a better choice because no high-power beam is needed, and the FED method can provide better resolution than STED when no high-power beam is allowed. The flexible switching of the two super-resolution imaging modes can help researchers to make most of the advantages of each imaging method. It is believed that the presented system has the potential to be widely used in future nanoscale investigations.

© 2015 Optical Society of America

Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy

P. Zhang, P. M. Goodwin, and J. H. Werner
Opt. Express 22(10) 12398-12409 (2014)

Resolution enhancement of saturated fluorescence emission difference microscopy

Guangyuan Zhao, Cuifang Kuang, Zhihua Ding, and Xu Liu
Opt. Express 24(20) 23596-23609 (2016)

Isotropic superresolution imaging for fluorescence emission difference microscopy

Shangting You, Cuifang Kuang, Zihao Rong, Xu Liu, and Zhihua Ding
Appl. Opt. 53(33) 7838-7844 (2014)

Super-resolution imaging of lateral distribution for the blue-light emission of an InGaN single-quantum-well structure utilizing the stimulated emission depletion effect

Yuichi Kozawa, Yuta Kusama, Shunichi Sato, and Hiroyuki Yokoyama
Opt. Express 22(19) 22575-22582 (2014)

Simultaneous super-linear excitation-emission and emission depletion allows imaging of upconversion nanoparticles with higher sub-diffraction resolution

Martin Plöschner, Denitza Denkova, Simone De Camillis, Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, Samuel Ojosnegros, and James A. Piper
Opt. Express 28(16) 24308-24326 (2020)