Abstract

This work presents cost-effective, simple arbitrary phase-step digital holographic microscopy to suppress both zero-order and twin-image terms. A virtual confocal offset lens under in-line configuration is also used to compensate for the introduced quadratic phase by using a microscope objective lens. In addition to reducing the difficulties of physical confocal configurations, the proposed method significantly increases the magnification power, ultimately achieving the purposes of an optical zoom. An attempt is also made to reduce the noise interference of a high magnification system by developing a long focal lens to reduce light detection size, subsequently gaining an approximately plane wave light source to illuminate the object within the effective depth of focus. Experimental results indicate that the proposed high magnification system can be elevated with low noise interference, and image reconstruction without quadratic phase terms.

© 2014 Optical Society of America

Multi-step phase aberration compensation method based on optimal principal component analysis and subsampling for digital holographic microscopy

Xiangyu Zhang, Jiasong Sun, Zuxin Zhang, Yao Fan, Qian Chen, and Chao Zuo
Appl. Opt. 58(2) 389-397 (2019)

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Tristan Colomb, Jonas Kühn, Florian Charrière, Christian Depeursinge, Pierre Marquet, and Nicolas Aspert
Opt. Express 14(10) 4300-4306 (2006)

Microlens characterization by digital holographic microscopy with physical spherical phase compensation

Qu Weijuan, Chee Oi Choo, Yu Yingjie, and Anand Asundi
Appl. Opt. 49(33) 6448-6454 (2010)

Simple and flexible phase compensation for digital holographic microscopy with electrically tunable lens

DingNan Deng, JunZheng Peng, WeiJuan Qu, Yu Wu, XiaoLi Liu, WenQi He, and Xiang Peng
Appl. Opt. 56(21) 6007-6014 (2017)

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Tristan Colomb, Etienne Cuche, Florian Charrière, Jonas Kühn, Nicolas Aspert, Frédéric Montfort, Pierre Marquet, and Christian Depeursinge
Appl. Opt. 45(5) 851-863 (2006)