Abstract

The light of a light-emitting diode or a common thermal source, such as a tungsten filament lamp, is known to be quasi-incoherent. We generated partially coherent light of these sources with a volume of coherence in the micrometer range of 5100μm3 by spatial and spectral filtering. The corresponding degree of partial coherence was adapted for microscopic interference setups, such as a digital in-line holographic microscope. The practicability of the sources was determined by the spectral emittance and the resulting signal-to-noise ratio (SNR) of the detector. The microscale coherence in correlation with the SNR and its resolution for microscopy were analyzed. We demonstrate how low-light-level, non-laser sources enable holographic imaging with a video frame rate (25frames/s), an intermediate SNR of 8 dB, and a volume of coherence of 3.4×104μm3. Holograms of objects with a lateral resolution of 1 μm were achieved using a microscope lens (50×/NA=0.7) and a CCD camera featuring a 4–12 bit dynamic range.

© 2012 Optical Society of America

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Supplementary Material (4)

» Media 1: MOV (3985 KB)     
» Media 2: MOV (3983 KB)     
» Media 3: MOV (4020 KB)     
» Media 4: MOV (4038 KB)     

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