Abstract

We investigated a high-precision optical method for measuring the thickness of biological samples regardless of their transparency. The method is based on the precise measurement of optical path length difference of the end surfaces of objects, using a dual-arm axial-scanning low-coherence interferometer. This removes any consideration of the shape, thickness, or transparency of testing objects when performing the measurement. Scanning the reference simplifies the measurement setup, resulting in unambiguous measurement. Using a 1310nm wavelength superluminescent diode, with a 65nm bandwidth, the measurement accuracy was as high as 11.6μm. We tested the method by measuring the thickness of both transparent samples and nontransparent soft biological tissues.

Full Article  |  PDF Article
OSA Recommended Articles
Simultaneous measurement of refractive index and thickness by combining low-coherence interferometry and confocal optics

Seokhan Kim, Jihoon Na, Myoung Jin Kim, and Byeong Ha Lee
Opt. Express 16(8) 5516-5526 (2008)

Optical coherence tomography system with no high-precision scanning stage and stage controller

Yu-Lung Lo, Chi-I Kuo, Chin-Ho Chuang, and Zhi-Zhong Yan
Appl. Opt. 43(21) 4142-4149 (2004)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription