Abstract
For research on inhomogeneous cells, we present a simulation method called the dual-medium quantitative (DMQ) measurement simulation method, which is realized by combining phase-shifting digital holography with DMQ analysis. The reliability of this method is confirmed by comparing the simulated phase map with the experimental one by the Hilbert phase microscope [J. Phys. Chem. A 113, 13327 (2009)], and its ability for studying inhomogeneous cells is demonstrated with measurements of a simulated HeLa cell. The average deviation and the relative deviation of physical thickness and axially averaged refractive index are , 0.69% and 0.0013, 0.094%, respectively. This approach can provide good guidance for experimental research on inhomogeneous cells.
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
Juanjuan Zheng, Peng Gao, Xiaopeng Shao, and G. Ulrich Nienhaus
Appl. Opt. 56(32) 9000-9005 (2017)
Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Yosuke Tamada, and Osamu Matoba
Appl. Opt. 59(24) 7144-7152 (2020)
Bhargab Das, Chandra S. Yelleswarapu, and D. V. G. L. N. Rao
Appl. Opt. 51(9) 1387-1395 (2012)