Dual-medium quantitative measurement simulation on cells

Abstract

For research on inhomogeneous cells, we present a simulation method called the dual-medium quantitative (DMQ) measurement simulation method, which is realized by combining phase-shifting digital holography with DMQ analysis. The reliability of this method is confirmed by comparing the simulated phase map with the experimental one by the Hilbert phase microscope [J. Phys. Chem. A 113, 13327 (2009)], and its ability for studying inhomogeneous cells is demonstrated with measurements of a simulated HeLa cell. The average deviation and the relative deviation of physical thickness and axially averaged refractive index are $0.0339\mathrm{\mu m}$, 0.69% and 0.0013, 0.094%, respectively. This approach can provide good guidance for experimental research on inhomogeneous cells.

© 2011 Optical Society of America