Abstract

We report a method of using optical aperture diffraction to simultaneously detect the image and the refractive index of a living cell. By simulation, it is found that, when a suitable gap is introduced between the cell and the aperture, the image of the cell on the detection plane can be amplified hundreds to thousands of times, and a limit of detection of 3e-4 to 9e-5 can be reached for the refractive index of the cell. Experiments show that this method is feasible to realize, but the achievement of such a detection system is yet to be proved.

© 2010 Optical Society of America

Algorithm and simulation for analysis of bioimages obtained by aperture diffraction based optical MEMS

Xiaodong Zhou, Daniel Puiu Poenar, Kai Yu Liu, Man Siu Tse, Chew-Kiat Heng, Swee Ngin Tan, and Nan Zhang
Opt. Express 16(16) 11937-11953 (2008)

Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution

Woo June Choi, Do In Jeon, Sang-Gun Ahn, Jung-Hoon Yoon, Sungho Kim, and Byeong Ha Lee
Opt. Express 18(22) 23285-23295 (2010)

Imaging the intracellular refractive index distribution (IRID) for dynamic label-free living colon cancer cells via circularly depolarization decay model (CDDM)

Huijun Wang, Lu Zhang, Jie Huang, Zewen Yang, Chen Fan, Li Yuan, Hong Zhao, Zhenxi Zhang, and Xiaolong Liu
Biomed. Opt. Express 15(4) 2451-2465 (2024)

Optical trapping and manipulation of live T cells with a low numerical aperture lens

John Harris and Gail McConnell
Opt. Express 16(18) 14036-14043 (2008)

Simultaneous measurement of group refractive index and thickness of optical samples using optical coherence tomography

Hsu-Chih Cheng and Yi-Cheng Liu
Appl. Opt. 49(5) 790-797 (2010)