Abstract

We describe the development and performance evaluation of a system for optical interrogation, subsequent selection, and collection of individual aerosol particles entrained in an inlet air stream. Elastic scatter and laser-induced fluorescence obtained from single particles on-the-fly provide compositional information for classification criteria. Individual particles could then be selectively electrically charged and captured to a conductive substrate with an electric potential. The optical subsystem also includes a novel two-beam velocimeter to provide accurate downstream timing. Good overall quantitative performance values are reported for particles in the size range of 18μm at mean rates up to 4kHz.

© 2009 Optical Society of America

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  1. D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
    [CrossRef] [PubMed]
  2. H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
    [CrossRef] [PubMed]
  3. R. H. Williams, I. E. Ward, and H. A. McCartney, “Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores,” http://www.pubmedcentral.nih.gov.
  4. J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
    [CrossRef]
  5. T. R. Unnasch, “Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment--permitting detection of pneumocystis carinii DNA,” Appl. Environ. Microbiol. 67, 449-452 (2001).
    [CrossRef] [PubMed]
  6. D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
    [CrossRef]
  7. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
    [CrossRef]
  8. M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
    [CrossRef]
  9. F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
    [CrossRef]
  10. Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
    [CrossRef]
  11. P. H. Kaye, J. E. Barton, E. Hirst, and J. M. Clark, “Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles,” Appl. Opt. 39, 3738-3745 (2000).
    [CrossRef]
  12. V. Sivaprakasam, A. Huston, C. Scotto, and J. Eversole, “Multiple UV wavelength excitation of bioaerosols,” Opt. Express 12, 4457-4466 (2004).
    [CrossRef] [PubMed]
  13. P. H. Kaye, W. R. Stanley, E. Hirst, E. V. Foot, K. L. Baxter, and S. J. Barrington, “Single particle multichannel bio-aerosol fluorescence sensor,” Opt. Express 13, 3583-3593 (2005).
    [CrossRef] [PubMed]
  14. Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
    [CrossRef]
  15. M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
    [CrossRef]
  16. V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.
  17. V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.
  18. T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).
  19. T. L. Pletcher, “System and method for spatially-selective particulate deposition and enhanced deposition efficiency,” U.S. patent 7,261,764 (28 August 2007)

2006 (1)

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

2005 (3)

P. H. Kaye, W. R. Stanley, E. Hirst, E. V. Foot, K. L. Baxter, and S. J. Barrington, “Single particle multichannel bio-aerosol fluorescence sensor,” Opt. Express 13, 3583-3593 (2005).
[CrossRef] [PubMed]

J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
[CrossRef]

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

2004 (3)

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

V. Sivaprakasam, A. Huston, C. Scotto, and J. Eversole, “Multiple UV wavelength excitation of bioaerosols,” Opt. Express 12, 4457-4466 (2004).
[CrossRef] [PubMed]

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

2003 (1)

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

2001 (1)

T. R. Unnasch, “Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment--permitting detection of pneumocystis carinii DNA,” Appl. Environ. Microbiol. 67, 449-452 (2001).
[CrossRef] [PubMed]

2000 (1)

1999 (3)

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

1995 (1)

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Barrington, S. J.

Barton, J. E.

Baxter, K. L.

Bottiger, J. R.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Boutou, V.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Bruno, J. G.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Cary,, W. K.

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Chang, R.

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

Chang, R. K.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Chen, B. T.

J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
[CrossRef]

Clark, J. M.

Datta, P.

T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).

Emanuel, P. A.

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

Eversole, J.

V. Sivaprakasam, A. Huston, C. Scotto, and J. Eversole, “Multiple UV wavelength excitation of bioaerosols,” Opt. Express 12, 4457-4466 (2004).
[CrossRef] [PubMed]

V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.

V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.

Eversole, J. D.

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Fernandez, G. L.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Foot, E. V.

Frain, M.

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

Halverson, J.

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Hardgrove, J. J.

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Hartings, J.

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Hill, S. C.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Hills, S. C.

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Hirst, E.

Huston, A.

V. Sivaprakasam, A. Huston, C. Scotto, and J. Eversole, “Multiple UV wavelength excitation of bioaerosols,” Opt. Express 12, 4457-4466 (2004).
[CrossRef] [PubMed]

V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.

V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.

Hyvarinen, A.

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

Jeys, T. H.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Kashon, M. L.

J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
[CrossRef]

Kaye, P. H.

Kearns, E. A.

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

Keswani, J.

J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
[CrossRef]

Kracke, S. K.

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

Kramer, M. F.

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

Lim, D. V.

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

Lin, H. B

V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.

Mayo, M. W.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

McCartney, H. A.

R. H. Williams, I. E. Ward, and H. A. McCartney, “Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores,” http://www.pubmedcentral.nih.gov.

McCoy, R. E.

T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).

Menking, D. E.

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

Nachman, P.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Nevalainen, A.

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

Newbury, N. R.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Pan, Y. L.

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Pan, Y.-L

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Paulin, L.

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

Pendleton, J. D.

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Pinnick, R. G.

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Pletcher, T. L.

T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).

T. L. Pletcher, “System and method for spatially-selective particulate deposition and enhanced deposition efficiency,” U.S. patent 7,261,764 (28 August 2007)

Poux, C. J.

T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).

Primmerman, C. A.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Reyes, F. L.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Rintala, H.

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

Roselle, D. C.

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Rowe, G. S.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Sanchez, A.

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Schmidt, D. P.

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

Schultz, A.

V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.

Scotto, C.

Seaver, M.

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Simpson, J. M.

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

Sivaprakasam, V.

V. Sivaprakasam, A. Huston, C. Scotto, and J. Eversole, “Multiple UV wavelength excitation of bioaerosols,” Opt. Express 12, 4457-4466 (2004).
[CrossRef] [PubMed]

V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.

V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.

Stanley, W. R.

Unnasch, T. R.

T. R. Unnasch, “Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment--permitting detection of pneumocystis carinii DNA,” Appl. Environ. Microbiol. 67, 449-452 (2001).
[CrossRef] [PubMed]

Valdes, J. J.

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

Ward, I. E.

R. H. Williams, I. E. Ward, and H. A. McCartney, “Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores,” http://www.pubmedcentral.nih.gov.

Williams, R. H.

R. H. Williams, I. E. Ward, and H. A. McCartney, “Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores,” http://www.pubmedcentral.nih.gov.

Wolf, J. P.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Zhang, S. S.

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

Aerosol Sci. Tech. (1)

R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, and J. G. Bruno, “Fluorescent particle counter for detecting airborne bacteria and other biological particles,” Aerosol Sci. Tech. 23, 653-664 (1995).
[CrossRef]

Aerosol Sci. Technol. (4)

M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Sci. Technol. 30, 174-185 (1999).
[CrossRef]

Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hills, J. Halverson, and R. K. Chang, “Single particle fluorescence spectrometer for ambient aerosols,” Aerosol Sci. Technol. 37, 628-639(2003).
[CrossRef]

Y. L. Pan, V. Boutou, J. R. Bottiger, S. S. Zhang, J. P. Wolf, and R. K. Chang, “A puff of air sorts bioaerosols for pathogen identification,” Aerosol Sci. Technol. 38, 598-602 (2004).
[CrossRef]

M. Frain, D. P. Schmidt, Y. L. Pan, and R. Chang, “Selective deflection and localization of flowing aerosols onto a substrate,” Aerosol Sci. Technol. 40, 218-225 (2006).
[CrossRef]

Appl. Environ. Microbiol. (1)

T. R. Unnasch, “Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment--permitting detection of pneumocystis carinii DNA,” Appl. Environ. Microbiol. 67, 449-452 (2001).
[CrossRef] [PubMed]

Appl. Opt. (1)

Clin. Microbiol. Rev. (1)

D. V. Lim, J. M. Simpson, E. A. Kearns, and M. F. Kramer, “Current and developing technologies for monitoring agents of bioterrorism and biowarfare,” Clin. Microbiol. Rev. 18, 583-607 (2005).
[CrossRef] [PubMed]

Field Anal. Chem. Technol. (1)

F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, and A. Sanchez, “Bio-aerosol fluorescence sensor,” Field Anal. Chem. Technol. 3, 240-248 (1999).
[CrossRef]

Indoor Air (1)

H. Rintala, A. Hyvarinen, L. Paulin, and A. Nevalainen, “Detection of streptomycetes in house dust-comparison of culture and PCR methods,” Indoor Air 14, 112-119 (2004).
[CrossRef] [PubMed]

J. Environ. Monitoring (1)

J. Keswani, M. L. Kashon, and B. T. Chen, “Evaluation of interference and real-time PCR for detection and quantification of fungi in dust,” J. Environ. Monitoring 7, 311-318 (2005).
[CrossRef]

Opt. Express (2)

Proc. SPIE (1)

D. E. Menking, S. K. Kracke, P. A. Emanuel, and J. J. Valdes, “Rapid cleanup of bacterial DNA from samples containing aerosol contaminants,” Proc. SPIE 3533, 215-219 (1999).
[CrossRef]

Other (5)

R. H. Williams, I. E. Ward, and H. A. McCartney, “Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores,” http://www.pubmedcentral.nih.gov.

V. Sivaprakasam, H. B Lin, A. Huston, and J. Eversole are preparing a paper called “Ambient aerosol and bio-threat agent simulant signature measured using dual wavelength excited fluorescence and elastic scatter” for submission to Opt. Express.

V. Sivaprakasam, A. Huston, A. Schultz, and J. Eversole are preparing a paper called “A polarized elastic scatter detection method to measure the velocity and track individual aerosol particles” for submission to Aerosol Sci. Technol.

T. L. Pletcher, P. Datta, C. J. Poux, and R. E. McCoy, “Method and apparatus for electrostatically depositing a medicament powder upon predefined regions of a substrate,” U.S. patent 6,007,630 (28 December 1999).

T. L. Pletcher, “System and method for spatially-selective particulate deposition and enhanced deposition efficiency,” U.S. patent 7,261,764 (28 August 2007)

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Figures (8)

Fig. 1
Fig. 1

Left, BioACE system showing the main system components. Right, a more detailed photo of the aerosol interrogation module (AIM) and charging and collection subsystem.

Fig. 2
Fig. 2

Schematic diagram of the optical layout of the AIM subassembly.

Fig. 3
Fig. 3

Aerosol chamber with optical collection elements.

Fig. 4
Fig. 4

Schematic diagram showing the optical layout of the depolarization measurement system for determining particle velocity.

Fig. 5
Fig. 5

Photographs of electrostatic sorting/deposition system using smoke visualization to illustrate aerosol particle trajectories during rejection (left) and collection (right).

Fig. 6
Fig. 6

Schematic illustration of the main data acquisition and processing electronics components and their interaction.

Fig. 7
Fig. 7

Screenshot of the FPGA illustrating the total and polarized elastic scatter signal and the ratio of the polarized to total scatter.

Fig. 8
Fig. 8

Microscope images obtained with a 10 × magnification objective show unsorted (left) and sorted (right) samples of collected PSL particles of a two-population aerosol mixture. Each population is identical in size ( 2 μm diameter), and the known blue to red ratio is 15 / 85 . In the sorted sample (right), the classification algorithm is set to collect the blue particles and reject the red particles.

Tables (2)

Tables Icon

Table 1 Static Capture Efficiency for 2, 5, and 8.1 μm Dye-Doped PSL Particles

Tables Icon

Table 2 Data Show Good Capture and Rejection Efficiencies Over a Wide Variation of Particle Throughput Rates and Aerosol Mixture Ratios

Metrics