Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy

Abstract

Total internal reflection (TIR) holographic microscopy uses a prism in TIR as a near-field imager to perform quantitative phase microscopy of cell–substrate interfaces. The presence of microscopic organisms, cell–substrate interfaces, adhesions, and tissue structures on the prism’s TIR face causes relative index of refraction and frustrated TIR to modulate the object beam’s evanescent wave phase front. We present quantitative phase images of test specimens such as Amoeba proteus and cells such as SKOV-3 and 3T3 fibroblasts.

© 2009 Optical Society of America

Digital holography of total internal reflection

William M. Ash III and Myung K. Kim
Opt. Express 16(13) 9811-9820 (2008)

Common-path configuration in total internal reflection digital holography microscopy

Alejandro Calabuig, Marcella Matrecano, Melania Paturzo, and Pietro Ferraro
Opt. Lett. 39(8) 2471-2474 (2014)

Amplitude and phase images of cellular structures with a scanning surface plasmon microscope

L. Berguiga, T. Roland, K. Monier, J. Elezgaray, and F. Argoul
Opt. Express 19(7) 6571-6586 (2011)

Refractive index profilometry using the total internally reflected light field

Tania Das and K. Bhattacharya
Appl. Opt. 56(33) 9241-9246 (2017)

Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages

Vishesh Dubey, Azeem Ahmad, Rajwinder Singh, Deanna L Wolfson, Purusotam Basnet, Ganesh Acharya, Dalip Singh Mehta, and Balpreet Singh Ahluwalia
Opt. Express 26(16) 19864-19876 (2018)