Nonlinear reconstruction of absorption and fluorescence contrast from measured diffuse transmittance and reflectance of a compressed-breast-simulating phantom

Abstract

We report on the nonlinear reconstruction of local absorption and fluorescence contrast in tissuelike scattering media from measured time-domain diffuse reflectance and transmittance of laser as well as laser-excited fluorescence radiation. Measurements were taken at selected source–detector offsets using slablike diffusely scattering and fluorescent phantoms containing fluorescent heterogeneities. Such measurements simulate in vivo data that would be obtained employing a scanning, time-domain fluorescence mammograph, where the breast is gently compressed between two parallel glass plates, and source and detector optical fibers scan synchronously at various source–detector offsets, allowing the recording of laser and fluorescence mammograms. The diffusion equations modeling the propagation of the laser and fluorescence radiation were solved in frequency domain by the finite element method simultaneously for several modulation frequencies using Fourier transformation and preprocessed experimental data. To reconstruct the concentration of the fluorescent contrast agent, the Born approximation including higher-order reconstructed photon densities at the excitation wavelength was used. Axial resolution was determined that can be achieved by various detection schemes. We show that remission measurements increase the depth resolution significantly.

© 2009 Optical Society of America

Three-dimensional time-resolved optical mammography of the uncompressed breast

Louise C. Enfield, Adam P. Gibson, Nicholas L. Everdell, David T. Delpy, Martin Schweiger, Simon R. Arridge, Caroline Richardson, Mohammad Keshtgar, Michael Douek, and Jeremy C. Hebden
Appl. Opt. 46(17) 3628-3638 (2007)

Three-dimensional unconstrained and constrained image-reconstruction techniques applied to fluorescence, frequency-domain photon migration

Ranadhir Roy and Eva M. Sevick-Muraca
Appl. Opt. 40(13) 2206-2215 (2001)

Optical mammography combined with fluorescence imaging: lesion detection using scatterplots

Anaïs Leproux, Marjolein van der Voort, Martin B. van der Mark, Rik Harbers, Stephanie M. W. Y. van de Ven, and Ton G. van Leeuwen
Biomed. Opt. Express 2(4) 1007-1020 (2011)

Subsurface diffuse optical tomography can localize absorber and fluorescent objects but recovered image sensitivity is nonlinear with depth

Dax S. Kepshire, Scott C. Davis, Hamid Dehghani, Keith D. Paulsen, and Brian W. Pogue
Appl. Opt. 46(10) 1669-1678 (2007)

Linear image reconstruction for a diffuse optical mammography system in a noncompressed geometry using scattering fluid

Tim Nielsen, Bernhard Brendel, Ronny Ziegler, Michiel van Beek, Falk Uhlemann, Claas Bontus, and Thomas Koehler
Appl. Opt. 48(10) D1-D13 (2009)