Abstract

Experimental methodology for imaging of laser-excited tissue autofluorescence bleaching rates has been developed and clinically tested. The fluorescence images were periodically captured from the same tissue area over a certain time, with subsequent detection of the fluorescence intensity decrease rate at each image pixel and further imaging the planar distribution of those values. Spectral features at each image pixel were analyzed with a hyperspectral imaging camera. Details of the equipment and image pro cessing are described as well as some measurement results that confirm the feasibility of the proposed technology.

© 2009 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Analysis of skin morphological features and real-time monitoring using snapshot hyperspectral imaging

Qinghua He and Ruikang K. Wang
Biomed. Opt. Express 10(11) 5625-5638 (2019)

Depth determination of chromophores in human skin by pulsed photothermal radiometry

Thomas E. Milner, Derek J. Smithies, Dennis M. Goodman, Alice Lau, and J. Stuart Nelson
Appl. Opt. 35(19) 3379-3385 (1996)

Fast nonlinear spectral microscopy of in vivo human skin

Arjen N. Bader, Ana-Maria Pena, C. Johan van Voskuilen, Jonathan A. Palero, Frédéric Leroy, Anne Colonna, and Hans C. Gerritsen
Biomed. Opt. Express 2(2) 365-373 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (12)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription