Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW. Labeling of Epstein–Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a short-term reporter molecule for human immunotherapy studies.

© 2008 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy

Gyungseok Oh, Su Woong Yoo, Yebin Jung, Yeon-Mi Ryu, Youngrong Park, Sang-Yeob Kim, Ki Hean Kim, Sungjee Kim, Seung-Jae Myung, and Euiheon Chung
Biomed. Opt. Express 5(5) 1677-1689 (2014)

In vivo photothermal treatment by the peritumoral injection of macrophages loaded with gold nanoshells

Taeseok Daniel Yang, Wonshik Choi, Tai Hyun Yoon, Kyoung Jin Lee, Jae-Seung Lee, Jang Ho Joo, Min-Goo Lee, Hong Soon Yim, Kyung Min Choi, Byoungjae Kim, Jung Joo Lee, Heejin Kim, Doh Young Lee, Kwang-Yoon Jung, and Seung-Kuk Baek
Biomed. Opt. Express 7(1) 185-193 (2016)

Optical clearing based cellular-level 3D visualization of intact lymph node cortex

Eunjoo Song, Howon Seo, Kibaek Choe, Yoonha Hwang, Jinhyo Ahn, Soyeon Ahn, and Pilhan Kim
Biomed. Opt. Express 6(10) 4154-4164 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (3)

» Media 1: AVI (1069 KB)     
» Media 2: AVI (1708 KB)     
» Media 3: AVI (3064 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription