Abstract

We describe a method to measure in vivo migration of human T cells by using the near-infrared (NIR) dye IRDye800CW. Labeling of Epstein–Barr virus-specific T cells with IRDye800CW did not affect viability, proliferation, or T cell function. Following tail vein injection into mice bearing subcutaneous tumors, the NIR signal could be measured in vivo at the tumor site. Analysis of tumors revealed T cell infiltration and an increased NIR signal, confirming T cell migration. To test specific migration with IRDye800CW, tumors were modified to express CCL5 to measure site-specific migration. The NIR signal was increased at CCL5-secreting tumors compared with control tumors. Together, these data suggest that IRDye800CW may be used to study the trafficking of T cells in a small animal model and may have potential as a short-term reporter molecule for human immunotherapy studies.

© 2008 Optical Society of America

In vivo fluorescence imaging of hepatocellular carcinoma xenograft using near-infrared labeled epidermal growth factor receptor (EGFR) peptide

Z. Li, Q. Zhou, J. Zhou, X. Duan, J. Zhu, and T. D. Wang
Biomed. Opt. Express 7(9) 3163-3169 (2016)

Quantitative pharmacokinetic and biodistribution studies for fluorescent imaging agents

Yichen Feng, Sanjana Pannem, Sassan Hodge, Cody Rounds, Kenneth M. Tichauer, Keith D. Paulsen, and Kimberley S. Samkoe
Biomed. Opt. Express 15(3) 1861-1877 (2024)

Relief of tumor hypoxia using a nanoenzyme amplifies NIR-II photoacoustic-guided photothermal therapy

Qiang Xue, Silue Zeng, Yaguang Ren, Yingying Pan, Jianhai Chen, Ningbo Chen, Kenneth K. Y. Wong, Liang Song, Chihua Fang, Jinhan Guo, Jinfeng Xu, Chengbo Liu, Jie Zeng, Litao Sun, Hai Zhang, and Jingqin Chen
Biomed. Opt. Express 15(1) 59-76 (2024)

High resolution combined molecular and structural optical imaging of colorectal cancer in a xenograft mouse model

Fabio Feroldi, Mariska Verlaan, Helene Knaus, Valentina Davidoiu, Danielle J. Vugts, Guus A. M. S. van Dongen, Carla F. M. Molthoff, and Johannes F. de Boer
Biomed. Opt. Express 9(12) 6186-6204 (2018)

Dynamic schema for near infrared detection of pressure-induced changes in solid tumors

Bei Wang, Stephen P. Povoski, Xianhua Cao, Duxin Sun, and Ronald X. Xu
Appl. Opt. 47(16) 3053-3063 (2008)