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Phase, amplitude, and polarization microscopy with a sampling field sensor

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Abstract

I describe an improved implementation of a previously reported interferometric device, the sampling field sensor (SFS) [Appl. Opt. 47, B32–B43 (2008)]. It provides X, Y, and XY shearing interferometric information simultaneously (space multiplexed) with amplitude and polarization information while using time-multiplexed phase shifting. Its simple common-path configuration makes it compact and vibration insensitive, as demonstrated by the λ/125 phase estimation repeatability that was below the coherent noise floor (estimated at λ/50). The SFS may be viewed as an efficient, robust and accurate full-field optical–digital interface, easy to integrate with traditional imaging systems. This is demonstrated by using the sensor as the focal plane array of a transmitted-light microscope in a straightforward setup using an illumination path polarization phase shifter. This work is focused on a qualitative demonstration and presents phase, amplitude, and polarization images of different types of human cheek cells and Caenorhabditis elegans larvae.

© 2008 Optical Society of America

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