Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging

Not Accessible

Your library or personal account may give you access

Abstract

Digital holography enables a multifocus quantitative phase microscopy for the investigation of reflective surfaces and for marker-free live cell imaging. For digital holographic long-term investigations of living cells an automated (subsequent) robust and reliable numerical focus adjustment is of particular importance. Four numerical methods for the determination of the optimal focus position in the numerical reconstruction and propagation of the complex object waves of pure phase objects are characterized, compared, and adapted to the requirements of digital holographic microscopy. Results from investigations of an engineered surface and human pancreas tumor cells demonstrate the applicability of Fourier-weighting- and gradient-operator-based methods for robust and reliable automated subsequent numerical digital holographic focusing.

© 2008 Optical Society of America

Full Article  |  PDF Article
More Like This
Digital holographic microscopy for live cell applications and technical inspection

Björn Kemper and Gert von Bally
Appl. Opt. 47(4) A52-A61 (2008)

Fast autofocusing in digital holography using the magnitude differential

Meng Lyu, Caojin Yuan, Dayan Li, and Guohai Situ
Appl. Opt. 56(13) F152-F157 (2017)

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Marquet, Benjamin Rappaz, Pierre J. Magistretti, Etienne Cuche, Yves Emery, Tristan Colomb, and Christian Depeursinge
Opt. Lett. 30(5) 468-470 (2005)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved