Abstract

We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digitalprocessing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object distorts the amplitude pattern according to its optical path length (OPL) profile, and the distorted pattern is recorded in a CCD detector. A digitalprocessing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients.

© 2007 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Structured illumination microscopy

Manish Saxena, Gangadhar Eluru, and Sai Siva Gorthi
Adv. Opt. Photon. 7(2) 241-275 (2015)

Structured illumination quantitative phase microscopy for enhanced resolution amplitude and phase imaging

Shwetadwip Chowdhury and Joseph Izatt
Biomed. Opt. Express 4(10) 1795-1805 (2013)

Efficient quantitative phase microscopy using programmable annular LED illumination

Jiaji Li, Qian Chen, Jialin Zhang, Yan Zhang, Linpeng Lu, and Chao Zuo
Biomed. Opt. Express 8(10) 4687-4705 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (38)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription