Abstract

A simplified approach is proposed to simulate the fluorescence signal from a fluorophore submerged inside a turbid medium using the Monte Carlo method. Based on the reversibility of photon propagation, the fluorescence signal can be obtained from a single Monte Carlo simulation of the excitation light. This is computationally less expensive and also allows for the direct use of well-validated nonfluorescence photon migration Monte Carlo codes. Fluorescence signals from a mouse tissuelike phantom were computed using both the simplified Monte Carlo simulation and the diffusion approximation. The relative difference of signal intensity was found to be at most 30% for a fluorophore placed in the medium at various depths and horizontally midway between a source–detector pair separated by 3  mm. The difference in time characteristics of the signal is also examined.

© 2007 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Depth resolution and multiexponential lifetime analyses of reflectance-based time-domain fluorescence data

Kenneth M. Tichauer, Mark Migueis, Frederic Leblond, Jonathan T. Elliott, Mamadou Diop, Keith St. Lawrence, and Ting-Yim Lee
Appl. Opt. 50(21) 3962-3972 (2011)

Sensitivity characterization of a time-domain fluorescence imager: eXplore Optix

Guobin Ma, Pascal Gallant, and Laura McIntosh
Appl. Opt. 46(10) 1650-1657 (2007)

Phantom and mouse experiments of time-domain fluorescence tomography using total light approach

Shinpei Okawa, Akira Yano, Kazuki Uchida, Yohei Mitsui, Masaki Yoshida, Masashi Takekoshi, Andhi Marjono, Feng Gao, Yoko Hoshi, Ikuhiro Kida, Kazuto Masamoto, and Yukio Yamada
Biomed. Opt. Express 4(4) 635-651 (2013)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (11)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription