UV fluorescence and absorption spectroscopy from Bacillus subtilis spores detected proteins and dipicolinic acid (DPA) released into the supernatant after heat treatments ranging from 20° to . The protein and DPA concentration in the supernatant was greater with higher heat treatment temperatures, undergoing a substantial increase for , and supporting the theory that spores undergo a phase transition from a glassylike to a rubberylike state at . Gel electrophoresis detected several small proteins with molecular weights between . These proteins may be small acid-soluble spore proteins that are present in spores but break down during germination. A 30 kDa protein extracted above is related to the rubber–glass phase transition.
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